Fig. 2. Expression pattern of Dnmt2. (A) Protein extracts were prepared from the developmental stages indicated and analyzed by western blotting. Significant amounts of Dnmt2 protein were found only in embryos. The position of marker proteins (in kDa) is indicated. A protein extract from adult females overexpressing Dnmt2 was included as an additional control for the specificity of our antibody (right lane). P40 signals are shown to indicate equal loading of all lanes. (B) Subcellular Dnmt2 localization in embryos. Stained embryos were analyzed by confocal microscopy. The protein is green, DNA is coloured red. Scale bars: 10 µm. (C) Depletion of Dnmt2 by RNA interference. Double-stranded RNA was injected into syncytial blastoderm embryos and embryos were stained after a 3 hour staging period. This procedure strongly reduced the amount of Dnmt2 protein.