(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. DOC1R mRNA and protein are present in mouse oocyte. (A) Expression of DOC1R mRNA in immature oocytes (lanes 1 and 2) and ovaries (lane 3), treated or not with reverse transcriptase (RT, + or -). (B) The anti-DOC1R antibody recognizes a band at 28 kDa in 200 oocytes arrested in metaphase II (lane 2) and recognizes 1 µg of purified DOC1R protein (lane 4). The antibody pre-incubated with the DOC1R peptide (peptide, + or -) does not recognize either endogenous DOC1R (lane 1) or purified DOC1R (lane 3). These experiments were repeated twice.