Fig. 2. Effect of suppression of macho-1 function by MO on formation of muscle and notochord. (A-C) Expression of the muscle myosin protein. (A) Control larva in which 300 pg of control MO was injected into fertilized egg. Anterior is towards the left. (B) 100 pg and (C) 300 pg of macho-1 MO was injected. (D-F) Expression of notochord-specific Not1 antigen. (D) Control tailbud embryo that was injected with 300 pg of control MO. (E) 100 pg and (F) 300 pg of macho-1 MO was injected. (B',C',E',F') Morphologies of embryos are shown in B,C,E,F, respectively. Scale bars: 100 µm.