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Fig. 6. Hrsna is downstream of macho-1 and inhibits notochord formation. (A) Expression of Hrsna at the 64-cell stage in embryos injected with mutant macho-1 mRNA (100 pg, control), macho-1 mRNA (100 pg) and macho-1 MO (100 pg). Anterior is upwards. (B) Morphology of the embryo injected with 100 pg of Hrsna mRNA. (C) Expression of notochord-specific Not1 antigen in cleavage-arrested 110-cell embryos injected with lacZ mRNA (100 pg, control) or Hrsna mRNA (100 pg). Anterior is upwards. (D) Expression of HrBra in the 110-cell stage embryos injected with lacZ mRNA (100 pg, control) or Hrsna mRNA (100 pg). Anterior is upwards. Scale bars: 100 µm.