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Fig. 2. Misexpressed CBF1 induces expression of SOHo1 and GH6, and represses expression of EphA3 and CBF2. Whole-mount in situ hybridization of E3 chick embryos (HH stage 18 to 20) transfected with CBF1/RCAS. Embryos were hybridized with antisense probes for SOHo1 (A,B), GH6 (C,D), EphA3 (E,F) or CBF2 (G,H). The untransfected sides (control) are shown in A,C,E,G and the contralateral transfected sides of the same embryo are shown in B,D,F,H as inverted images for ease of comparison. Arrowheads indicate the border of the endogenous expression area on the control side.