Fig. 1. Localisation of LRC in different compartments of wild-type mouse epidermis. Epidermal sheets (A-H) or tissue sections (I) were labelled for keratin 14 (red; A-E; H-I) or Ki67 (red; F,G) and BrdU (green) by double-label immunofluorescence. Skin was collected from tail (A-D,F-I) or back (E) of adult wild-type mice. (A) One hour BrdU label. (B-I) Mice received repeated injections of BrdU to generate LRC. Wholemounts prepared following a chase of 2 days (B) or 140 days (C). Asterisk in C indicates site of insertion of dermal papilla. (D,E) Epidermis from 42-day-old mouse (telogen; 31 days post-BrdU injection). (F-I) Epidermis collected following a 70 day chase period. (F) Arrows indicate LRC located in the interfolliclular epidermis. (G) Hair follicle plucked from labelled wholemount. Arrowhead indicates a LRC in the infundibulum. Inset is higher power view of boxed area, showing two cells double-labelled for Ki67 and BrdU. Cells with intense (BrdU heavy) and weak (BrdU light) BrdU labelling are indicated. (H) High power confocal micrograph of sebaceous gland; arrows indicate the position of LRC. (I) Hair follicle in tissue section; arrows indicate keratin 14-positive LRC in the outer root sheath. HF, hair follicle; IFE, interfollicular epidermis; SG, sebaceous gland; BG, bulge. Scale bars: 100 µm.