Fig. 2. Localisation of cell lineage markers and putative stem cell markers in tail wholemounts from adult mice. (A-J) Tail epidermal wholemounts from wild-type mice were immunolabelled to detect the proteins indicated or stained with Nile Red (D) to visualise sebocytes. (E) Bracket shows lack of labelling of HF outer root sheath from below the sebaceous gland to the bulb. (J) Brackets show that labelling for keratin 15 is present within the bulge region of hair follicles. Inset is a higher power view of boxed area, showing double-label immunofluorescence for keratin 15 (red) and BrdU (green) in a mouse labelled according to the LRC protocol followed by a chase of 32 days. Staining of sebaceous glands is non-specific because of the use of a mouse primary antibody. (K,L) High-power micrographs of IFE labelled by double-label immunofluorescence for keratin 10 (green) and either ß1 integrin (red; K) or 6 integrin (red; L). BG, bulge; HF, hair follicle; IFE, interfollicular epidermis; SG, sebaceous gland; CCL, companion cell layer. Scale bars: 100 µm in A-J; 20 µm in K-L.