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Fig. 2. Immunohistochemical localization of ERBB4 expression. Paraffin wax-embedded number 4 inguinal mammary glands from Erbb4+/+Wap-Cre (A,C,E) and Erbb4Flox/FloxWap-Cre (B,D,F) mice, at L1 (A,B), L14 (C,D) and P18 of the second pregnancy (E,F), were stained for ERBB4 expression by IHC. Nuclear ERBB4 protein can be detected in Erbb4+/+Wap-Cre mammary glands and Erbb4Flox/FloxWap-Cre mammary glands at L1. Membrane staining of ERBB4 at L14 is indicated by the arrowhead in C and stromal expression is indicated by the arrow in D. Scale bar: 50 µm.