Development -- Long et al. 130 (21): 5257 Figure IG6

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 6. Erbb4^Flox/FloxWap-Cre secretory epithelium fails to express the differentiation marker NPT2B. Immunohistochemical staining of NKCC1 (red) and SMA (green) in mammary glands of biparous Erbb4^+/+Wap-Cre and Erbb4^Flox/FloxWap-Cre mice, at P13 (A,B), P18 (C,D) and L1 (E,F). Immunohistochemical staining of NPT2B (red) and ß-catenin (green) in mammary glands of biparous Erbb4^+/+Wap-Cre and Erbb4^Flox/FloxWap-Cre mice, at P13 (G,H), P18 (I,J) and L1 (K,L). Lack of NPT2B staining in Erbb4^Flox/FloxWap-Cre mammary glands at L1 indicates a defect in epithelial differentiation. Arrowheads (A,B,E) indicate NKCC1 staining and arrowheads (I,K) indicate NPT2B staining of the apical surface of secretory epithelium.