Fig. 5. Correlation of ventral cord defects in various mutants. (A-C) Wild type with PVP and PVQ axon labeled in different colors. (A,B) Single channel, (C) overlay. (D-F) sax-3(ky123) mutant with PVP and PVQ axon labeled in different colors. (D,E) Single channel, (F) overlay. The PVPR axon (D) and the PVQL axon (E) cross the midline repeatedly at the same positions (arrowheads). (G-I) lin-11(n389) mutant with PVQ and interneuron axons labeled differentially. (G,H) Single channels, (I) overlay. PVQL and interneurons cross into the contralateral axon tract at different positions (arrowheads in G and H). (J-L) nid-1(ur41) mutant with PVP and PVQ axon labeled in different colors. (J,K) Single channels, (L) overlay. The AVG and PVPL axons (J) and the PVQR axon (K) cross the midline at the same position (arrowheads) to run together in the left axon tract. (M-O) unc-6(ev400) mutant with PVP/AVG and D-type motoneuron axons labeled differentially, (M,N) Single channels, (O) overlay. The AVG axon crosses the ventral midline to grow in the left axon tract (arrowhead in M), whereas the D-type motoneuron axons all grow out correctly on the right side. All animals are shown from a ventral aspect with anterior towards the left. Scale bars: 10 µm in A-I,M-O; 20 µm in J-L. Markers shown are glr-1::GFP (green in H,I), unc-47::DsRed (red in N,O), odr-2::CFP (light blue in B,C,E,F,J,L,M,O), sra-6::DsRed2 (yellow in A,C,D,F,G,I,K,L).