Fig. 3. Localization of PAR-2 and HMR-1/E-cadherin. (A-D) Each row shows a single 8-cell embryo, either wild-type or par-3(ZF1), immunostained for both PAR-3 (A,C) and PAR-2 (B,D). (B',D') higher magnifications of the boxed regions in B,D, respectively. The arrow in C points to PAR-3 that remains in a young somatic cell. Note presence of apical PAR-2 in the par-3(ZF1) embryo (arrow in D'). (E) Two cells in an 8-cell par-3(ZF1) embryo immunostained for PAR-3 (red) and HMR-1 (green). PAR-3 is present at the apical cortex (arrow) of one cell only. Note that HMR-1 is present at basolateral surfaces but is not detected at apical surfaces (arrowhead). DNA is stained blue in all panels. Yellow asterisks mark germline precursors. Scale bar: 5 µm.