Fig. 4. Localization of apical PAR proteins. Each row depicts a single embryo immunostained for the protein at the bottom right of each panel. Embryo genotypes are shown above each row. Where visible, the germline precursor is indicated with a yellow asterisk and the youngest somatic precursors are indicated by arrows. Embryos are shown at representative stages; similar results were obtained for other stages. (A-B) An 8-cell embryo. (C-D) A 12-cell embryo. (E-F) A 14-cell embryo. (G-H) A 26-cell embryo. At this stage, PAR-3^ZF1-GFP is present only in the three youngest somatic cells (arrows), whereas apical PAR-3 is present in several older somatic cells (arrowheads in H).