Fig. 7. The cardiac fate map and RALDH2. (A,C) Grids were superimposed on bright field/fluorescent overlays of HH7 and HH8 embryos injected with DiI in the lateral mesoderm, respectively. (B,D) Bright field/fluorescent overlays of embryos depicted in A and C at HH11⁺, respectively. (A) DiI injected in the lateral mesoderm at the level of Hensen's node. (B) HH11⁺. DiI injected in A was located in atrium and left sinus venosus. (C) DiI injected at the anterior lateral mesoderm. (D) HH11⁺. DiI injected in C was located in left and right ventricles (white arrowheads). (E,F) Fate maps of embryos at stages HH7 and HH8 respectively superimposed on typical Raldh2 expression patterns. (E) HH7. Chick Raldh2 expression predicts the location of prospective sino-atrial precursors. (F) HH8. Anterior and posterior cardiac precursors occupy distinct territories, but chick Raldh2 expression no longer discriminates anterior from posterior cardiac precursors. (G,H) HH7 and HH8 fate maps data grouped as anteroposterior (AP) divisions. (I) The cardiac fate map at stage HH8 was superimposed on a typical Gata4 expression pattern.