Fig. 3. The role of Spitz in rotation and evidence that the Egfr acts directly. (A,B) Section through adult eye of spi^scp1 hypomorph. Many ommatidia show under-recruitment defects; misrotations, however, are very rare (green trapezoid). Colour coding in schematics is as for previous Figures (black, correctly orientated; green, underrotated; blue, overrotated; black circles, mis-specified ommatidia; red line: equator). (C) Quantification of rotational defects in spi^scp1 versus ru¹ and S/+ versus spi^scp2. `No.' indicates the number of eyes scored for each genotype. In spi^scp1, very few misrotations are seen relative to the proportion of misrecruitments; the converse is seen in ru¹. However, spi^scp2 dominantly enhances rotational defects of S/+, suggesting Spitz plays some role in the control of rotation. (D-F) S/+; svp-lacZ/+ 40 hour pupal retina stained with -cut (D; red in F), -lacZ (E; blue in F) and -Elav (green in F). Ommatidial orientation and cone cell number are not correlated: ommatidia with too few cone cells may be either correctly (solid circle) or incorrectly (broken circle) orientated, and incorrectly orientated ommatidia may also have the correct number of cone cells (dotted circle).