(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. Eye discs misexpressing Keren are indistinguishable from wild type (WT). All images show eye discs taken from crawling third instar larvae. In all cases, green is {alpha}-Elav, marking photoreceptors. Upper panels show the red channel alone; lower panels are merges. (A D) m{Delta}0.5-lacZ staining (red) in WT (A,B) and UAS-keren (C,D) discs. m{Delta}0.5-lacZ highlights the R4 cell and acts as a marker for chirality; R4 determination is normal in UAS-keren discs. (E-H) {alpha}-Bar staining (red), highlighting R1 and R6 in WT (E,F) and UAS-keren (G,H) discs. Rare misrotations can be seen in both WT and mutant discs (arrowheads). (I,L) svp-lacZ (red) staining in WT (I,J) and UAS-keren (K,L) discs. seven-up is expressed strongly in R3 and R4 (outer pair in each ommatidium) and weakly in R1 and R6 (inner pair). Note the similarity between WT and UAS-keren with all three markers.