Fig. 5. Rotational defects arise during pupal eye development. All images are confocal projections of svp-lacZ/+ retinae stained with -Elav (green) and -ß-galactosidase (red). Upper panels show the red channel only; lower panels are merges. svp-lacZ highlights R1,3,4 and 6. (A-D) Discs taken from wild type (WT) (A,B) and UAS-keren (C,D) flies at 6 hours post-pupariation. At the back of the WT disc, ommatidia are arrested at 90°. UAS-keren discs show no disruption in rotation, even at the back of the disc where ommatidia are oldest. (EH) Retinae taken from WT (E,F) and UAS-keren (G,H) flies at 30 hours post-pupariation. By this stage, rotational defects are clear in the UAS-keren retinae (compare E with G).