Fig. 2. Expression of Wit in Tv neurons but not the NHO is able to rescue FMRFa
expression. Rescue of a wit null heteroallelic animal by
elav>Gal4 (A, w; P{UAS>wit,
w+}/P{elav>Gal4, w+};
witA12, st/witB11, st) or C929>Gal4
(B, w; P{UAS>wit, w+}/P{C929>Gal4,
w+}; witA12, st/witB11,
st) driving UAS>wit. Note rescue of FMRFa expression in the
NHO and Tv neurons. (A,B) The third pair of Tv neurons is not in the plane of
focus. (C-F) Expression of nuclear lacZ using the 929>Gal4 driver
is shown in green, while FaRP is in red. (C) Focused on the NHO. Note the lack
of lacZ staining in the NHO. (D) Tv neurons showing FMRFa expression (E) The
same plane of focus as in D showing nuclear lacZ. Yellow arrows
identify Tv neuron nuclei. (F) The merged image showing that the C929>Gal4
driver expresses in Tv neurons. Scale bars: in A 100 µm for A,B; panel C 30
µm; panel D (for D-F) 50 µm.