Fig. 3. Cell-autonomous rescue of FMRFamide expression by Wit. Larvae of the genotype w; P{UAS>witGFP, w⁺}/P{OK6>Gal4, w⁺}; wit^A12, st/wit^B11, st were stained for FMRFamide and Wit (GFP). z-stacks are shown. (A-C) Dorsal view with anterior on top, showing the chimeric rescue of FMRFamide expression in Tv neurons. The three (out of six possible) FMRFamide-positive Tv neurons are marked by yellow arrows (A). Note the predominantly motoneuron GFP staining that reports driver expression. (D-F) High magnification view of the frame in B. Note co-expression (yellow in E) of WitGFP (F) and FMRFamide (D). (B,E) Merged images. Scale bar: 100 µm in A-C; 20 µm in D-F. Dorsal view, anterior upwards.