Fig. 4. Mutations in wit do not affect apterous expression or Tv neuron viability. Third instar larval brains are shown with GFP in green and anti-FMRFamide in red. FMRFamide staining in Tv and subesophageal neurons is indicated by yellow and blue arrows, respectively. (A-F) w; P{aP>Gal4, w⁺}/{UAS>GFP, w⁺}; wit/TM6B. (D-F) Higher magnification of the area framed in B. (G-L) w; P{aP>Gal4, w⁺}/{UAS>GFP, w⁺}; wit^A12/wit^B11 animals. (J) High-magnification view of the area framed in G. (K,L) High-magnification views of Ap-positive clusters in two other mutant animals. Scale bar: 100 µm for A-C,G-I; 20 µm for D-F,J-L.