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Fig. 6. Gbb is required for FMRF expression. (A) in situ hybridization of third instar brain and ventral ganglia with a gbb probe. The arrows indicate the NHO. (B-E) FMRF expression in (B) wild-type, (C) gbb1/gbb4 hypomorph and (D) gbb1/gbb2 null mutant animals. (E) Rescue of FaRP expression in a gbb-null background by expression of Gbb using the neuron specific elav>Gal4 driver (w; P{UAS>gbb, w+}, gbb1/gbb2; P{elav>Gal4, w+}/+), (F) Lack of FaRP rescue in a gbb null background using the muscle specific G14>Gal4 driver to express Gbb (w; P{UAS>gbb, w+}, gbb1/gbb2, P{G14>Gal4, w+}). Scale bars: in A, 50 µm for A; in B, 100 µm for B-F Anterior towards the left, dorsal views.