Fig. 9. Expression of FMRFa in neuroendocrine cells enables wit mutants to
eclose without rescuing the primary defects in synaptic transmission or
synapse morphology. (A) Wild-type Oregon R. (B-D) Partial rescue of
wit adults in which FMRFa is expressed in neuroendocrine cells using
the C929 driver (w; P{UAS>FMRFa,
w+}/P{C929>Gal4, w+};
witA12, st/witB11, st). (B) Partial
eclosion, (C) full eclosion but no wing inflation, and (D) full eclosion and
full wing inflation. (E) Lack of wing inflation in adult expressing
Gl
in neuroendocrine cells. (F) Wild-type synapse at muscles 6/7 visualized by
anti-Csp staining. (G) Small synapse at muscles 6/7 of
witA12/witB11 animals expressing FMRFa with the
C929 driver. (H) Representative traces of evoked junctional potentials of wild
type, wit mutant and wit mutant expressing FMRFa. (I)
Histogram representation of 5 recordings. Scale bar: 20 µm.