Fig. 3. TrkB (full length and truncated), NT4 and BDNF are expressed in mouse ovaries at E16, P0 and P4. Full-length TrkB is expressed at very low levels in all ovary samples. Each RT-PCR reaction was carried out on P0 brain and on ovary at E16.5, P0 and P4. RT-PCR was carried out for: (1) cyclophilin; (2) full-length TrkB using equivalent amounts of mRNA (in terms of cyclophlin expression); (3) full-length TrkB receptor with eight times more E16 ovary mRNA than brain mRNA and five times more P0 and P4 ovary mRNA than brain mRNA (in terms of cyclophlin expression); (4) TrkB receptor, both truncated and full length, using equivalent amounts of mRNA (in terms of cyclophlin expression); (5) NT4, using equivalent amounts of mRNA (in terms of cyclophlin expression); and (6) BDNF using equivalent amounts of mRNA (in terms of cyclophlin expression).