Fig. 5. Dominant-negative eomes inhibits dorsal expression of gsc and flh. (A-D) The dorsal blastoderm margin of embryos at 50% epiboly was dissected and flat-mounted. Bottom right corner indicates in situ probe used, top right corner indicates gene construct, if injected. (A) Expression of gsc in an uninjected embryo. (B) Injection of eomes-eng inhibits gsc expression. Eomes-eng expressing cells shown in brown by anti-GFP antibody staining. (C) Expression of flh in an uninjected embryo. (D) Injection of eomes-eng inhibits flh expression. Eomes-eng expressing cells shown in brown by anti-GFP staining.