Fig. 2. Expression of CCR1 protein on human EVTs in the cell column at 10-weeks gestation. To determine the differentiation stage of EVTs that express CCR1 in the cell column, serial sections of placental tissue from therapeutic hysterectomy at 10-weeks gestation were either single-immunostained with anti-integrin 1 mAb followed by FITC-conjugated secondary antibody (B) or double-immunostained with anti-CCR1 mAb followed by rhodamine-conjugated secondary antibody plus FITC-conjugated anti-integrin 5 mAb (C-E). Trophoblasts are visualized by staining with anti-cytokeratin 7 mAb (A). The cytotrophoblast layer is traced with a dashed line. Two adjacent cell columns (Column 1 and Column 2) are shown. In these cell columns, the initial expression sites of CCR1 (D, arrowheads) are similar to those of integrin 5 (C,E). Note that CCR1 expression is diminished on migrating interstitial trophoblasts (Int TB) (D,E) that express both integrin 1 (B) and integrin 5 (C,E). AV, anchoring villus; Gl, decidual gland. Scale bars: 100 µm.