Fig. 3. Expression of CCR1 protein on human endovascular trophoblasts at 10-weeks gestation. Serial sections of placental tissue from therapeutic hysterectomy at 10-weeks gestation were double-immunostained with anti-CCR1 mAb followed by rhodamine-conjugated secondary antibody plus either FITC-conjugated anti-cytokeratin 7 mAb or FITC-conjugated anti-von Willebrand factor pAb to visualize trophoblasts (B,E) and blood vessels (C,F), respectively. Endovascular trophoblasts (Endov TB) near the intervillous space (IVS) (A-C) and those in the deep portion of the decidual tissue (D-F) are shown. Expression of CCR1 is detected on endovascular trophoblasts that have just entered the maternal artery from the trophoblastic shell (Shell) (C), whereas it is hardly detected on the endovascular trophoblasts that reside in the maternal arteries located in the deep portion (F). Gl, decidual gland. Scale bars: 200 µm.