Fig. 4. Differential expression of chemokine receptor mRNAs in the isolated human EVTs. mRNA was extracted from EVTs isolated from explant cultures of chorionic villi at 8-weeks gestation and RT-PCR performed using specific primers against chemokine receptors CCR1-11, CXCR1-5, XCR1, CX3CR1 and S26. cDNA from PBMCs was used as a positive control (top panel). The isolated EVTs express CCR1, CCR10 and XCR1 (middle panel). The negative control (total RNA from isolated EVTs without reverse transcription) is shown in the bottom panel.