Fig. 3. Patch clamp analysis of ESGPs before and after transplantation. (A-C) Cultured ESGPs exhibit I_K(A) and/or I_K(D) but lack I_Kir and I_K(P). The current pattern during propagation in EGF/FGF (A), PDGF/FGF (B), and 9 days after growth factor withdrawal (C) are shown. (D-F) After transplantation, ESGPs have three distinct current patterns. Whereas some cells retain the immature pattern observed during monolayer culture (D), others display I_Kir (E). The majority of engrafted ESGPs displayed prominent I_K(P) (F). Current phenotypes shown in E,F are characteristic of mature hippocampal astrocytes in vivo. The pulse protocol (see text) used in A,B also applies to (C, left) and (D-F).