Development -- Yang et al. 130 (23): 5569 Figure IG3

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Fig. 3. PP2A:B56 ${epsilon}$ is required for Wnt/ß-catenin signaling. (A) Western blot showing endogenous cytoplasmic ß-catenin in maternal PP2A:B56 ${epsilon}$ -depleted embryos at stage 9. hn-RNP was used as a loading control. (Shown here is a representative result from three different experiments). (B) RT-PCR showing that injection of mRNA encoding VP16-Xtcf3 (2 pg), ß-catenin (500 pg), GID (1 ng), and Xdsh (2 ng) rescue siamois and Xnr3 expression in maternal PP2A:B56 ${epsilon}$ -depleted embryos at stage 10, while Wnt8b (20 pg) does not. ODC was used as loading control. Wnt8b (20 pg), Xdsh (2 ng), GID (1 ng), ß-catenin (500 pg), and VP16-Xtcf3 (2 pg) induce similar percentages of secondary axis when injected ventrally into normal embryos at the 4-cell stage (not shown).