Fig. 6. In ovo misexpression of Six3 suppressed diencephalic Wnt3 and Gbx2 expression and upregulated Dlx2 in the dT. (A-F) Serial sections of embryos 48 hours after electroporation with Six3-IRES-GFP at HH stage 9-10. In sections in which GFP was visualized in the dT (A), Gbx2 expression was suppressed (B, n=8). In 25% of these embryos Dlx2 was induced ectopically in the dT (arrowheads in C). Misexpression of Six3-IRES-GFP in the dT (D) also results the down-regulation of Wnt3 (E,F, n=2). Scale bar: 100 µm.