Fig. 9. Manipulating XSOX3 accumulation. (A) Morpholinos against the 5' untranslated region (5' UTR) and coding sequence of XSOX3 and XSOX7 were generated and compared with the XSOX3 RNA sequence. (B) Upon injection into fertilized eggs, the XSOX3 morpholino led to a decrease in XSOX3 polypeptide level in embryos analysed at stage 9; the injection of the XSOX7 morpholino had no apparent effect on XSOX3 accumulation. Neither morpholino effected the accumulation of XTCF3 (recognized by the anti-XTCF3n antibody). (C) Embryo lysates were prepared and subject to quantitative RT-PCR analysis. Depletion of XSOX3 led to an increase in Xnr5 RNA compared with uninjected and XSOX7-morpholino-injected embryos.