Fig. 4. Sox9 induces neural crest markers but not RhoB. All panels show embryos electroporated with Sox9 and analysed at the indicated times after transfection; images are oriented with the transfected side of the neural tube to the right. Slug (A-C) and Cad6B (D-F) are induced transiently by Sox9; upregulation can be detected at 6 hpt (A,D) but expression levels have returned to basal by 12 hpt and 24 hpt (B,C,E,F). (G-I) Ectopic Cad7 expression is observed at all time points and ectopic expression of Cad7 is observed in cells migrating away from the neural tube (I, red arrow). (J-L) Ectopic FoxD3 expression is also detected in transfected neural tube but not until 24 hpt (L). Sox10 expression is normal at 6 hpt (M) but ectopic induction can be detected at 12 hpt (N) and continues at 24 hpt (O). By contrast, RhoB is not induced in any time point examined (P-R) and reduction of endogenous expression is observed at 6 hpt (P), 12 hpt (Q) and 24 hpt (R).