Fig. 5. LET-502 and MEL-11 immunolocalization in wild-type embryos. Embryos in the first column are stained with anti-LET-502, middle column with anti-MEL-11 and merged images are presented in the right column (LET-502 red, MEL-11 green). (A-C) 1.0- to 1.5-fold embryo showing extensive colocalization of LET-502 and MEL-11 in all epidermal cells. (D-F) Twofold embryo is shown at a plane corresponding to the epidermal cells where MEL-11 is restricted to epidermal cell boundaries while LET-502 remains cytoplasmic. (G-I) Twofold embryo at a plane corresponding to pharynx and intestine, where LET-502 and MEL-11 colocalized at adherens junctions. (J-L) Threefold embryo showed cytoplasmic LET-502, while MEL-11 showed punctate staining at the cell boundaries, with a limited amount reappearing in the cytoplasm (compare E and K). All images were deconvolved. Scale bars: 10 µm.