Fig. 4. Low nuclear Medea accumulates in dorsolateral cells during late gastrulation. (A) Confocal projection of Medea staining in a stage 8 embryo, with regions of high magnification, single optical sections (B-D) indicated with yellow boxes. Medea immunofluorescence in white (A) or green (B, B',C,C',D,D') and Topro3 DNA stain in red (B',C' and D'). Nuclei stained with Topro3 are diffuse red with bright spots of condensed chromatin. (A). At the midline, nuclei have higher levels of Medea than the cytoplasm (B,B'). Medea is largely excluded from nuclei in ventral ectoderm cells (C,C'; dorsal here because of germband extension). In dorsolateral cells there is an even distribution of Medea between the cytoplasm and the nucleus. This shoulder of low signal is absent anterior to the cephalic furrow. B-D were collected at the same gain. Anterior is leftwards.