Fig. 6. Both BMP ligands are required for nuclear SMAD responses. Confocal projections of stage 6/7 embryos stained for Medea (A,A',C,C') or PMAD (B,B',D,D'), dorsolateral views except C',D' are dorsal. Mutants at left (A-D), with gain-matched wild-type (WT) controls at right (A'-D'). For each mutant-WT pair (e.g. A and A'), contrast was manipulated coordinately; contrast and confocal gain differ between different pairs. (A,B) In dpp^H46 embryos, neither Medea nor PMAD is detectable in nuclei in any region. (C) In scw^s12 embryos, Medea does not accumulate in nuclei, although apical staining is slightly more intense in approximately the dorsal 40% of the embryo. (D) PMAD levels are at background in somatic cells of scw^s12 mutants. Anterior is leftwards.