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Fig. 1. G{alpha} and Gß mutants display opposite phenotypes and the Gß phenotypes are due to gain of G{alpha} activity. (A) DIC images of live one-cell embryos recorded by time-lapse video microscopy in various genetic backgrounds (as indicated) undergoing the first division. Black arrowheads indicate the current position of centrosomes in each image. White arrowheads indicate the position of the centrosome in the previous image during anaphase spindle pole oscillations. White arrows indicate multiple nuclei. Relative time points are indicated (minutes:seconds). Note the rapid changes in centrosome position in gpb-1(RNAi) embryos (rocking), and the lack of anaphase spindle pole oscillations in G{alpha} mutant embryos. (B) Spherical G{alpha}(RNAi) embryos. Anterior is towards the left in this and all subsequent figures. Scale bar: 10 µm.