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Fig. 3. Localization of GPR-1/2. Confocal sections of wild-type (A-I), gpr-1/2(RNAi) (J), G{alpha}(RNAi) (K-O) and gpb-1(RNAi) (P-T) embryos stained with anti GPR-1/2 antibodies (top panels for each series) and DAPI to visualize DNA (bottom). (A,P) One-cell prophase embryos. (B,K,Q) One-cell metaphase embryos. (C,D,L) One-cell anaphase embryos. (E) One-cell telophase embryo. (F,J,M,R) Two-cell interphase embryos. (G) Two-cell prophase embryo. (H) Two-cell embryo where P1 is at metaphase. (I,N,O,S,T) Four-cell interphase embryos. White arrowheads indicate the boundaries of the domains enriched GPR-1/2. Scale bar: 10 µm.