Fig. 4. GPR-1/2 asymmetry depends on PAR-3 and LET-99. (A-N) Confocal images of wild-type embryos (A-D), par-3 embryos (E-I) and let-99 embryos (J-N) stained with anti-GPR-1/2 antibodies (top panels) and DAPI (bottom panels). (A,E,J) One-cell prophase embryos. (K) One-cell metaphase embryos. (B,F,L,M) One-cell late anaphase embryos. (C,G,N) Two-cell interphase embryos. (D,H,I) Four-cell interphase embryos. (O) Confocal images of wild-type and gpr-1/2(RNAi) anaphase embryos stained with anti-LET-99 antibodies. Scale bar: 10 µm. (P) Quantification of relative intensity of GPR-1/2 staining in wild-type, gpb-1(RNAi) and let-99(or81) one-cell prophase embryos.