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Fig. 4. Early neural crest defects in lowts213 mutants. Two-color, whole-mount in situ hybridization was performed at the six-somite (A-F) or 12-somite (G-L) stages, with antisense RNA probes to (A-D) foxd3 and ctn, (E,F) ctn alone, (G,H) sox9b, and (I-L) sna2 and ctn in wild-type (A,C,E,G,I,K) and lowts213 mutant (B,D,F,H,J,L) embryos. Lateral (A,B,F,I,J) and dorsal (C,D,G,H,K,L) views, anterior towards the left. Arrows indicate the otic vesicle. e, eye; mb, midbrain; hb, hindbrain; r3-6, rhombomeres 3-6; sc, spinal cord. Scale bars: 100 µm.