Fig. 5. Defects in pigment precursors, neuronal and glial derivatives of the neural crest. (A-H) Whole-mount in situ hybridization with riboprobes to dct (A,B) and kit (C,D) at 22 hpf (lateral views), as well as gch (E,F) at 25 hpf (dorsal views) and foxd3 (G,H) at 28 hpf (lateral views) in wild-type (A,E,C,G) and low^ts213 mutants (B,D,F,H). (I-L) In situ hybridization for isl1 in wild type (I,K) and mutants (J,L) at the six-somite stage (dorsal views). (M-R) Immunostaining with anti-Hu antibody at 72 hpf (lateral views) of wild type (M,O,Q) and mutants (N,P,R) showing cranial (M,N) and spinal (O,P) ganglia (arrows), and enteric neurons (Q,R, arrows). (S,T) Immunostaining with anti-TH antibody of carotid bodies (arrows) at 60 hpf (ventral views) in wild type (S) and mutant (T). e, eye; mn, motoneurons; n, notochord; ot, otic vesicle; rb, Rohon-Beards, V, trigeminal ganglia, X, vagal ganglia. Scale bars: 100 µm.