Fig. 6. Defects in neural crest migration and survival in low^ts213 mutants. (A-D) Living 24 hpf embryos labeled by laser activation of DMNB-caged fluorescein (green) 12 hours earlier in the dorsal midbrain and posterior hindbrain of wild type (A) and low mutant (B), or anterior hindbrain of wild type (C) and low mutant (D), and corresponding neural crest (arrows). (E,F) TUNEL labeling of dying cells in six-somite stage zebrafish embryos. (G,H) Co-labeling with TUNEL and in situ hybridization for ctn mRNA at the eight-somite stage (dorsolateral views just posterior to the otic vesicle, ot) showing dying cells within the normal ctn expression domain and in adjacent non-neural ectoderm (arrows). (I) Histogram comparing numbers of apoptotic cells in wild type (blue) and low^ts213 mutants (red). Scale bars: 100 µm.