Fig. 2. Coincidence of AO and VGAL signals in the epidermis. A wild-type embryo was injected with AO and VGAL. (A-I) A series of images that are projections of five 2 µm optical sections from a time-lapse recording of the lateral epidermis. The embryo is shown at (A-C) 1/2 germband retraction (GBR), 3/4 GBR (D-F) and full GBR (G-I). (A,D,G) AO fluorescence; (B,E,H) VGAL fluorescence; (C,F,I) both fluorescence channels. A gray mask was applied to cover the amnioserosa.