Fig. 2. The vab-19 gene encodes a conserved ankyrin repeat-containing protein. (A) Genetic and physical maps of the vab-19 region and structure of the vab-19 gene. All rescue experiments used vab-19(e1036) and the rol-6 (pRF4) co-injection marker. Initial experiments assayed rescue of the morphological defects of e1036 at the semipermissive temperature 22.5°C. Rescue was classified as follows: no rescue (injection of pRF4 alone) means that 20% of Rol animals are Vab; weak rescue (+) means that 5-10% Rol animals are Vab; partial rescue (++) means that 2-5% Rol animals are Vab; full rescue (+++) means that <2% animals are Vab. All rescuing transgenes also rescue the lethal Vab-19 phenotypes. (B) Comparison of C. elegans VAB-19, human Kank/KIAA0172, mouse NG28 and Drosophila CG10249, isoform C. A second murine VAB-19 homolog is represented by an apparent partial sequence (AAH06647, not shown). The predicted effects of vab-19 mutations are indicated. The four ankyrin repeats are shown as striped boxes. Sequence alignments suggest that the four highly conserved repeats might be flanked by partial or divergent ankyrin repeats (not shown). (C) ClustalW alignments of VAB-19 family N-terminal motifs A and B.