Fig. 7. The effect of PI-PLC treatment on migration of GFP⁺ neurons in cortical slices. (A) Tag1 immunostaining of a cortical slice incubated in a control solution for 5 hours. (B) Tag1 immunostaining of a cortical slice after 2 hours incubation in a PI-PLC-supplemented solution. No immunostaining can be observed. (C) The time sequence of migrating neurons in an E15.5 coronal slice taken over a time interval of 60 minutes. (D,E) The relationship between the position of GFP⁺ neurons in the cerebral cortex and their direction of migration or the rate of migration. (D) The average speed of migration for individual cells plotted against the distance from the ventricular surface. (E) The direction of migration as measured from deflection from a line drawn vertical to the ventricular surface was plotted against the distance from the ventricular surface. See legend to Fig. 4 for further explanation. Scale bars: 125 µm in A,B; 50 µm in C.