Fig. 1. The amphioxus Hairy subfamily. (A) Alignment of the amphioxus Hairy proteins conducted by ClustalW. Black boxes represent identical residues among the Amphi-Hairy proteins. The bHLH, the orange and the C-terminal WRPW domains are indicated. (B) Neighbour-joining phylogenetic tree relating the amphioxus Hairy proteins with other selected Hairy proteins from vertebrate and protostome species. The tree is rooted using the human and the mouse Hes2 sequences. The numbers refer to the bootstrap values. c, chicken; Dm, Drosophila melanogaster; h, human; m, mouse; Tc, Tribolium castaneum; X, Xenopus laevis; zf, zebrafish.