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Fig. 2. Linkage of Amphi-hairyA to Amphi-hairyF genes in the amphioxus genome. DNA from PAC 51K5 was subjected to PCR reactions with Amphi-Hairy specific oligonucleotides. Amphi-Hairy A to Amphi-Hairy F (lines A to F) gave positive bands of the expected sizes (Amphi-hairyA, 300 bp; Amphi-hairyB, 156 bp; Amphi-hairyC, 311 bp; Amphi-hairyD, 249 bp; Amphi-hairyE, 235 bp; Amphi-hairyF, 207 bp), indicating that they are contained in the same genomic region.