Fig. 2. Bar is required for downregulation of Ato expression. (A-C) Ato expression is strongly elevated within Bar loss-of-function clones (A). Bar mutant cells are marked by the loss of GFP staining (B). Bar mutant cells showed ubiquitous upregulation of Ato expression (C, white arrow) and individually singled-out Ato⁺ cells (C, yellow arrow). (D) dpp-Gal4 drives lacZ expression in the dorsoventral lateral margins of the eye disc and in the ventral region of the antennal disc (arrow). (E,F) Misexpression of BarH1 by dpp-Gal4 strongly downregulates Ato expression. The furrow progression was strongly decreased in the ventral margin region of the eye disc where dpp drives BarH1 expression (E, arrowhead). Ato expression in the ventral region of antennal disc was dramatically downregulated (E,F, arrows).