Fig. 6. Transcriptional repression of ato by Bar is Ci^FL-independent. (A-D) Third instar eye-antennal disc of hh^P30 (enhancer trap) (Ma et al., 1993) was stained with antibodies for ß-gal (Hh-lacZ; A, blue), Ci^FL (A,B,D, red) and Ato (C; A,D, green). The expression domains of Ci^FL and Ato approximately overlap within the furrow (A,D). (E-H) Complementary expression pattern of Ci^FL and Bar. Wild-type third instar eye-antennal discs were stained with antibodies for Ci^FL (E,F,H, red) and BarH1 (E,G,H, green). (I-L) Bar loss-of-function clone (I,L, loss of GFP staining) shows no increase in Ci^FL expression (J). Note that the same clone shows elevated expression of Ato (K).