Fig. 2. Shot colocalizes with microtubules and the microtubule + end-binding proteins EB1 and CLIP190 in the fusion track. Anterior, leftwards; dorsal, upwards. (A,C,E,G) Before fusion; (B,D,F,H) after fusion. (A-H) Composited 1 µm confocal sections of stage 13 wild-type embryos. (A) Prior to fusion, a track (arrowhead) containing microtubules (red), Shot (green) and EB1 (blue) forms in fusion cells. The same proteins also colocalize apically (arrow). Merge of C, E, G. Scale bar: 10 µm. (B) The fusion track (arrowhead) contains CLIP190 (blue), as well as microtubules (red) and Shot (green, merge of D,F,H). (C,D) Microtubules in the fusion track (arrowhead). (E,F) Shot in the fusion track (arrowhead). (G) EB1 in the fusion track (arrowhead). (H) Clip 190 in the fusion track (arrowhead).