Fig. 4. Transient activation of proneural genes following Ebf1 misexpression. Stage HH10-HH12 chick embryos were co-electroporated on the right side with Ebf1 and GFP expression plasmids, collected 10 hours (A-C) or 20 hours (D-F) later, and processed for in situ hybridisation with the indicated probes. The pictures show flat mounts of the anterior region of the spinal cord. The cases shown are representative of more than 90% of the embryos, from three independent experiments, each involving at least eight embryos per probe.