Fig. 6. In situ hybridization analysis of mouse Gcm1. Coronal sections of E14.5 mouse forebrains were hybridized with a digoxigenin-labeled mouse Gcm1 antisense riboprobe (A,B). (B) Higher magnification of the boxed area in A; inset shows higher magnification of the boxed area in B. Dispersed distribution of mouse Gcm1 mRNA was observed in the ganglionic eminence and in the thalamus. Only a few signals lining the ventricular zone were observed in the cerebral cortex. Sections were counterstained with Methyl Green after in situ hybridization. As controls, sections of E11.5 placenta were hybridized with a digoxigenin-labeled mouse Gcm1 antisense (C) and sense (D) riboprobes, respectively. mouse Gcm1 expression was observed only in labyrinth layer of placenta using antisense riboprobe. No signal was observed in embryonic brain sections (not shown), or in placenta sections (D). Scale bars: 150 µm in A-D; 50 µm inset of B.